FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6356081
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329700.5187158565159884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228050.3587902671473192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156680.24650409584144697No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71340.11223897241082988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100050.073.646861
AAGTACG3650.065.33111
TCTGTCG41850.059.4178738
TGCGGAC3050.056.5632639
GTATCAA223300.055.182911
TCTCGCT39600.053.336032
GTCTCGC35550.053.3261681
CTCGCTC37500.053.3083883
CGCTCTG38300.050.170325
CAACGCA292450.044.5080765
ATCAACG292400.044.4591983
TCAACGC297250.043.7538074
AACGCAG305550.042.891236
ACGGGTC9550.042.363623
TATCAAC307700.042.1518362
ACGGGAC13650.041.843323
TCTGTCA61500.041.110258
TCGCTCT54600.040.7536474
GTCTCAC61450.040.4545171
ACGCAGA325350.040.262697
Produced by FastQC (version 0.11.5)