FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6356081
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110170.17333007556071106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTC37150.055.1094743
GTCTCGC40200.055.0615921
TCTCGCT40650.053.4301642
TCTGTCG45000.053.4086888
GGTATCA60050.053.0673331
CGCTCTG40600.048.2344475
GTATCAA125750.047.2852551
GTCTCAC65400.045.187421
TCGCTCT54150.042.0825584
CAACGCA138250.042.022995
TCTGTCA65650.041.309598
TGCGGAC3800.040.6040539
ACGCAGA144150.040.549697
AACGCAG144800.040.367986
GTACGGG287550.040.242711
ATCAACG146350.040.0617753
CGCAGAG145800.040.0504078
GGCTAGA33350.040.0361821
TCACTCT86100.039.4241454
TCAACGC148900.039.2962954
Produced by FastQC (version 0.11.5)