FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7241548
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414560.5724742831228903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264510.3652672052991984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238350.329142332550996No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133000.18366238820760422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115950.070.466541
TCTGTCG45400.056.6128588
GTATCAA255600.055.3182871
GTCTCGC42200.055.1375471
TCTCGCT46500.052.5899852
CTCGCTC45550.051.988693
CGCTCTG44000.050.709555
TGCGGAC3900.048.8171659
ATCAACG343650.042.765623
CAACGCA348300.042.17735
TCGCTCT59350.042.1058774
TCAACGC350950.041.9777874
AACGCAG360900.040.8508956
TATCAAC360150.040.69072
GGCTAGA34400.040.3070141
GTCTCAC65200.038.699151
ACGGGAC15100.038.6156463
GTACGGG310700.037.8467671
ACGCAGA389600.037.8263327
CGCAGAG392150.037.580368
Produced by FastQC (version 0.11.5)