FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6467912
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199010.3076881689175734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG40250.056.1306238
GTCTCGC38500.052.828131
TCTCGCT38650.051.70472
CTCGCTC35750.051.5734143
CGCTCTG36050.048.9994745
GGTATCA71100.047.174721
GTATCAA139650.044.2886281
ACGGGAC17750.043.5597343
GTACGGG314250.041.198651
GTCTCAC54500.040.5925451
CAACGCA149150.039.9961245
GGCTAGA29750.039.380311
ACGGGTC8450.038.712053
TCGCTCT53100.038.5304574
ATCAACG155300.038.2590563
AACGCAG156450.038.076836
ACGCAGA156150.038.0735447
TCAACGC157950.037.8430944
CGCAGAG157050.037.7432568
CTTGTCG4500.037.003721
Produced by FastQC (version 0.11.5)