FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6450602
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420410.6517376207677981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258940.4014198984838934No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189030.29304241681629095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179760.27867166506319874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119350.065.666941
TCTGTCG42800.060.191248
GTCTCGC37150.057.5067751
CTCGCTC36650.055.849683
GTATCAA256750.055.6963461
TCTCGCT39250.055.3336682
CGCTCTG36050.054.467175
ACGGGAC15800.046.321683
ATCAACG318450.045.815823
CAACGCA324700.044.7129865
TCAACGC326300.044.6953664
AACGCAG335400.043.4425056
TCGCTCT51750.043.0028764
GTCTCAC61650.042.5685581
TAAGCCC15450.042.364342
GGCTAGA30650.042.1320571
TATCAAC349250.041.7924082
GTACGGG287800.041.1477131
ACGCAGA356500.040.8712927
CGCAGAG359600.040.5189558
Produced by FastQC (version 0.11.5)