FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6450602
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207810.32215597862029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC36400.055.053641
TCTGTCG41400.054.4208078
TCTCGCT38200.051.3770032
CTCGCTC36600.050.8613853
GGTATCA71350.050.4218641
CGCTCTG37850.047.6103825
ACGGGAC16600.045.142683
GTATCAA138850.043.2118451
GTACGGG314400.040.1158181
GAGTCGG8950.039.8644451
GTCTCAC57800.039.6086851
CAACGCA148450.039.5422945
ATCAACG153200.038.471563
GGCTAGA33850.038.1204951
AACGCAG155200.037.9990736
GTACGAG3450.037.919371
TCAACGC154950.037.8838274
ACGCAGA157300.037.530157
TCGCTCT53500.037.463164
CGCAGAG159250.037.0303968
Produced by FastQC (version 0.11.5)