FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6484807
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421930.6506438819227773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266510.41097599358007103No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178680.27553634209930994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177860.2742718480287848No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG64860.1000183968466602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118800.066.447821
TCTGTCG41750.058.4185878
GTCTCGC37950.056.8729821
GTATCAA257950.056.513551
CTCGCTC37200.054.3826143
TCTCGCT39700.053.9555472
CGCTCTG37900.050.5425385
TGCGGAC3250.049.4201479
ATCAACG320150.045.998733
CAACGCA324650.045.3707165
TCAACGC326700.045.094714
AACGCAG334050.044.1991546
GTCTCAC55600.043.322731
TGTCGCG1109.642463E-943.2734382
TATCAAC350300.042.0056882
ACGGGAC15300.042.000693
ACGCAGA355800.041.5641447
CGCAGAG359150.041.2427028
GTACGGG283350.041.052851
GGCTAGA32500.040.1763081
Produced by FastQC (version 0.11.5)