FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7757808
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497270.640992919649468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296060.3816284187492137No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222810.28720741735294303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166700.21488028577144472No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA90510.11666955408022472No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78460.101136815966572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133550.071.148721
GTATCAA290400.058.330661
GCGTAAC2450.055.8557431
GCGTACT4550.054.9217031
TCTGTCG40900.051.782098
GTCTCGC36550.050.1382751
CAACGCA355400.047.7260065
CTCGCTC35050.047.5275343
ATCAACG359300.047.2907563
TCAACGC365000.046.6337434
TCTCGCT38550.046.2990572
AACGCAG368500.046.125946
GCGTAAG7550.044.9194031
TATCAAC377200.044.888852
CGCTCTG37500.043.6291475
GCCCGTT16050.043.3696673
ACGCAGA396650.042.8674137
CGCAGAG400250.042.4502038
CCCGTTG16600.041.932724
CGTACTT5400.039.6628572
Produced by FastQC (version 0.11.5)