FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7760852
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497270.6407415062160701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299740.3862204819780096No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223070.2874297821940168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167370.21565931163227955No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA90810.11701034886375877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130200.069.786921
GTATCAA290200.058.3352171
GCGTACT4950.051.690321
TCTGTCG41900.050.8358578
GTCTCGC35200.050.2064741
GCGTAAC2150.049.817281
CTCGCTC34750.047.774413
CAACGCA358500.047.3182685
ATCAACG360650.047.1214073
GCGTAAG9300.046.7074171
TCAACGC368350.046.2638244
AACGCAG367800.046.2006076
TATCAAC372300.045.3272252
TCTCGCT39000.044.551692
CGCTCTG36850.043.4345745
ACGCAGA398950.042.3546457
CGCAGAG402300.042.0019578
GCCCGTT17500.041.4827733
CCCGTTG17750.041.2321434
CGTACTT5700.040.7132452
Produced by FastQC (version 0.11.5)