FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7755057
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT496630.6403950351364277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294330.3795329937613611No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221670.2858392917034652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162250.20921832038113972No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA88930.11467356074881205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128400.069.796011
GCGTACT5150.061.2405781
GTATCAA289650.059.8257941
GCGTAAC1900.056.375391
TCTGTCG40600.050.1178328
GTCTCGC38250.049.9395071
GCGTAAG7750.049.1415561
CAACGCA354850.048.9119155
ATCAACG359250.048.3797263
TCAACGC365700.047.6240584
CGTACTT6000.047.601282
AACGCAG367650.047.2703936
CTCGCTC38350.046.546233
TATCAAC374850.046.0647282
TCTCGCT42050.045.988162
CGCTCTG37300.045.303585
ACGCAGA400950.043.35937
CGCAGAG405500.042.8581058
GCAGAGT448400.038.8240629
CCCGTTG17250.038.632924
Produced by FastQC (version 0.11.5)