FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7755057
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223050.2876187757227316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG38300.048.895678
GTATCAA143000.048.0372731
GGTATCA70500.047.5797771
GTCTCGC35500.047.2447051
CTCGCTC34600.044.6940083
TCTCGCT39950.042.7278982
CAACGCA162750.041.1873055
CGCTCTG37100.039.7592545
AACGCAG171050.039.416886
ACGCAGA171150.039.359627
CGCAGAG173050.038.9926648
ATCAACG175150.038.576273
TCAACGC177150.037.9736144
CCCGTTG15800.037.6446574
GCCCGTT15600.037.364013
GTCTCAC69650.034.7540741
CTGTCGC54900.033.894839
TATCAAC203150.033.4931222
TCGCTCT51050.033.4384844
GCAGAGT204450.032.742579
Produced by FastQC (version 0.11.5)