FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7834325
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT496910.6342728952398579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299400.3821643855724648No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212360.27106355684759054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188750.240926946482307No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA91820.11720218397883672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134700.071.8281251
GTATCAA299350.058.508121
TCTGTCG43450.051.481158
GCGTACT3150.049.204681
CAACGCA366050.047.814635
GTCTCGC36050.047.459181
ATCAACG370450.047.204563
GCGTAAC2150.047.1361471
TCAACGC377550.046.4429324
AACGCAG377800.046.3896376
TATCAAC385550.045.3249362
CTCGCTC36500.043.5243573
ACGCAGA408050.042.8923157
CGCAGAG411850.042.496568
TCTCGCT39600.042.2206652
CGCTCTG37300.041.468955
GCAGAGT453150.038.6890759
CTGTCGC59250.038.3552869
GCCCGTT15900.037.0469133
GTACTTT551700.036.533041
Produced by FastQC (version 0.11.5)