FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7834325
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259590.3313495419196932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC37000.051.4887431
TCTGTCG41550.050.9941148
GTATCAA150300.047.8972741
CTCGCTC37450.047.7008863
GGTATCA74100.047.334551
TCTCGCT41300.045.6971172
CGCTCTG39450.043.478695
CAACGCA165950.042.1659775
AACGCAG174000.040.1808326
ATCAACG175450.040.0168723
ACGCAGA176150.039.6230137
CGCAGAG175800.039.6006248
TCAACGC179100.039.1363144
TCGCTCT53400.036.5662354
CTGTCGC60150.035.8187529
GTCTCAC64750.035.2882351
GCCCGTT13600.034.4746553
TATCAAC205550.034.330182
GCAGAGT206000.033.6235169
CCCGTTG14250.032.903184
Produced by FastQC (version 0.11.5)