FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5628039
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123660.2197212919100241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG38900.055.7775768
TGCGGAC3100.055.6089829
GTCTCGC35150.053.632991
CTCGCTC32150.052.7235343
TCTCGCT36800.050.5867962
GGTATCA54600.049.231591
CGCTCTG33800.048.3905455
GTATCAA112600.045.9493031
GTCTCAC55100.042.524841
CAACGCA121150.041.729195
TCGCTCT44600.040.939654
AACGCAG126700.040.0192956
ATCAACG125400.039.9825633
TCAACGC127300.039.5726934
ACGCAGA128700.039.3963437
GTACGGG266100.039.266751
CGCAGAG130250.038.975938
ACGGGAC14950.038.589683
GGCTAGA27650.038.0694161
CTGTCGC58150.037.6189549
Produced by FastQC (version 0.11.5)