FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5628668
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378870.6731077405880042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209900.3729123835337242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165070.29326654192430607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131380.23341223891691606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91950.068.599961
TCTGTCG40500.059.055428
GTATCAA208300.058.993011
GTCTCGC39850.057.6406331
TCTCGCT41500.056.78282
CTCGCTC39250.056.550823
CGCTCTG39000.054.1634675
TGCGGAC2900.051.289779
TCGCTCT50600.047.8628124
GTCTCAC53200.047.5386771
ATCAACG259800.047.390483
CAACGCA263400.046.7850465
TCAACGC265100.046.5087174
AACGCAG270450.045.6934246
ACGGGAC12500.044.749443
TATCAAC280400.043.9725342
ACGCAGA290100.042.4130257
GGCTAGA31050.042.3545841
CGCAGAG292950.042.019978
TCTGTCA59750.041.721978
Produced by FastQC (version 0.11.5)