FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5663971
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150760.26617367920845636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG39400.058.586728
GTCTCGC37400.056.0058751
CTCGCTC35750.054.276253
TCTCGCT39350.053.2314262
TGCGGAC3600.052.7478529
CGCTCTG36950.050.1055645
GGTATCA53300.047.5369341
GTATCAA108850.045.355021
ACGGGAC13750.044.450093
TCGCTCT50000.041.8935054
CTGTCGC58500.039.8652049
CAACGCA119750.039.8406455
GTACGGG260050.039.360691
ATCAACG123750.038.887843
AACGCAG125700.038.143286
TCAACGC125650.038.1112374
ACGCAGA126150.038.101297
GTCTCAC55750.037.9973951
CGCAGAG126500.037.9962128
GGCTAGA30000.037.1849371
Produced by FastQC (version 0.11.5)