FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5077274
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359680.708411639789383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199640.39320312435373783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181080.3566480753254601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75600.063.5923351
GTATCAA157600.056.7437481
ATCAACG192000.048.4026763
TCAACGC192300.048.3890464
CAACGCA195450.047.5178535
AACGCAG200250.046.378396
TATCAAC215300.043.385562
CGCAGAG225950.041.073658
ACGCAGA225750.041.0605747
GCAGAGT260300.035.722019
CGACCGT504.4419328E-429.74900232-33
AGAGTAC255400.028.91857710-11
CAGAGTA271650.026.27983510-11
GAGTACT240550.025.88123312-13
CGTCAAT5750.024.8329726
AGTACTT265050.024.26324812-13
GTGGTAT45400.023.3281021
AGTACGG42950.022.57748212-13
GAGTACG40800.021.94458812-13
GTACTTT297350.020.99740214-15
Produced by FastQC (version 0.11.5)