FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5077274
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA32450.039.58955
TCAACGC32850.039.4691544
ATCAACG35100.037.1085243
AACGCAG35700.036.1302536
CGCAGAG36600.035.5673148
ACGCAGA37550.034.349867
GTATCAA53400.028.3991641
CGCGGCA2650.024.69261752-53
TACGGGT7800.024.4001244
ACGCGGC2750.023.78824250-51
GAGTACG38150.023.3831671
AGTACGG39000.022.7226162
ACGGGAC3506.548362E-1122.0908263
ACACGCG3100.022.05743248-49
GTACGGG46950.022.0404421
CACGCGG3050.021.44841650-51
GCAGAGT62100.021.1542349
GCAGCGC2909.784526E-820.4969888
TATCAAC67650.020.4844722
TACGGGG26100.020.280852
Produced by FastQC (version 0.11.5)