FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5061503
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361210.7136417779461951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200550.39622618024725065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179810.3552502092757823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70400.062.72481
GTATCAA154650.058.1463661
ATCAACG184200.050.393983
TCAACGC184500.050.2475364
CAACGCA186150.049.6423235
AACGCAG192200.048.166396
TATCAAC206850.045.105992
CGCAGAG216250.042.8371358
ACGCAGA216800.042.7284627
GCAGAGT252200.036.8724379
CGTCAAT5050.032.9878926
AGAGTAC246250.029.1741910-11
CAGAGTA260600.026.38052610-11
GAGTACT231800.025.93697712-13
AGTACTT251700.025.1391612-13
GCAGCGC2502.0136213E-823.7984098
AGTACGG39900.023.4137212
GAGTACG38600.023.1266121
CTGTCGC5200.022.8830839
CGGGACT2352.910947E-722.7886354
Produced by FastQC (version 0.11.5)