FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5061503
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA31350.045.147275
TCAACGC31750.044.7657934
ATCAACG32950.043.496443
AACGCAG34250.041.654956
CGCAGAG33950.041.4932338
ACGCAGA36000.039.134687
GTATCAA55250.029.4886251
CGCGGCA2750.027.01902452-53
GTCGTGC2308.349161E-925.8528481
GAGTACG39450.025.4727571
ACGCGGC2850.025.0292650-51
AGTACGG39800.024.952912
TACGGGT7050.023.6189734
GCAGAGT60500.023.3829299
TATCAAC65750.022.9734272
TAGACCG2102.805824E-622.6549345
CTGTCGC5850.022.3534289
CACGCGG3300.021.61618250-51
GTCTCGC4750.021.2809751
TCCCCGC7050.021.0775268
Produced by FastQC (version 0.11.5)