FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5061624
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC32950.040.5248264
CAACGCA33450.039.20945
ATCAACG34850.038.8255423
AACGCAG35100.037.3654826
CGCAGAG35050.037.2494748
ACGCAGA36550.035.8827827
GTATCAA51050.029.991981
GTCTCGC4650.025.5245571
CGCGGCA2650.024.7674752-53
ACGCGGC2750.023.85913350-51
CACGCGG2900.022.6250450-51
GAGTACG38700.022.61998412-13
AGTACGG39700.022.0502112-13
TATCAAC66000.021.490152
GCAGAGT63650.020.9784749
CCGGTCG3151.0481017E-820.723929
TACGGGT8400.020.4886234
ACACGCG3400.020.12493748-49
TCCCCGC6850.019.926128
GTACGGG47150.019.7605551
Produced by FastQC (version 0.11.5)