FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5298334
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356570.6729851307977186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205160.3872160569718708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159990.3019628434145526No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62700.11833908545591879No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71400.070.8317641
GTATCAA160350.062.6340981
ATCAACG201350.050.7352263
TCAACGC201750.050.6346364
CAACGCA202100.050.458635
AACGCAG210650.048.437456
TATCAAC228100.044.7853472
ACGCAGA238800.042.702687
CGCAGAG239150.042.6128928
GCAGAGT268900.037.964759
TCTGTCG13200.034.7033048
GTCTCGC12350.030.3515511
AGAGTAC272200.028.64197310-11
CAGAGTA282350.027.37003710-11
CTGTCGC17550.025.762659
GAGTACT268950.025.10604912-13
GCAGCGC4850.024.5325288
TCTCGCT14950.024.2761342
AGTACTT281250.024.21960312-13
GCAGCGT3800.023.486321
Produced by FastQC (version 0.11.5)