FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5266734
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA35400.038.3044055
GTCTCGC12300.036.741371
TCAACGC37650.036.6471444
ATCAACG38150.036.009583
CGCAGAG38450.034.485038
ACGCAGA39400.034.412037
CTCGCTC11850.034.1264533
TCTGTCG15050.033.5818448
AACGCAG40900.033.2963186
GTATCAA45450.032.7079241
TCTCGCT14700.030.342312
GGTATCA25750.030.0201591
CTGTCGC19450.026.5938039
CGCTCTG14500.026.2499875
GCAGCGC4800.026.013588
GAGTACG44200.023.812111
GTACGGG53900.023.3880481
AGTACGG46650.022.0545982
GCAGAGT61200.021.5666359
TACGGGA25150.020.8089122
Produced by FastQC (version 0.11.5)