FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5274697
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350370.6642466856390045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203660.38610748636367165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156510.29671846553460796No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61850.11725792021797651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.074.993791
GTATCAA164850.063.1023331
ATCAACG206100.051.5346833
TCAACGC207500.051.1869774
CAACGCA207550.051.0011865
AACGCAG216050.049.0180176
TATCAAC233550.045.9871752
ACGCAGA246650.042.9849667
CGCAGAG247550.042.7565848
TCTGTCG14550.042.526578
GCAGAGT277300.038.212379
CGCTCTG12900.037.3613325
GTCTCGC12150.036.245021
CTCGCTC13650.033.5658343
CTGTCGC17950.033.4770289
TCTCGCT15050.032.8156472
GCAGCGC4100.030.4737178
AGAGTAC278150.027.94604310-11
CAGAGTA290050.027.92767310-11
GAGTACT273850.025.43085912-13
Produced by FastQC (version 0.11.5)