FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5274697
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA37950.042.1573945
TCAACGC41050.039.4084174
ATCAACG42300.038.5247043
CGCAGAG42350.038.1831328
ACGCAGA42900.037.6961067
GTATCAA47850.037.1583631
AACGCAG43750.036.6915866
TCTGTCG15550.032.1147778
GTCTCGC13150.029.3937441
GCAGCGC4100.027.5502668
GCAGAGT61250.026.4969649
CTCGCTC14550.025.7517473
GTACGGG57600.024.5709421
TCTCGCT16050.024.0859242
CTGTCGC20450.023.8374679
GAGTACG46350.023.6067071
GGTATCA30350.023.5120161
CGCTCTG15750.022.6570825
AGTACGG50000.022.59014512-13
TATCAAC78350.021.7096122
Produced by FastQC (version 0.11.5)