FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4953845
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409660.8269536087624865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198450.4005979193939253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152370.3075792641877168No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA79920.16132923012326789No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC75530.15246742681694725No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC66810.13486493824493903No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG66280.13379506221934678No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT63400.12798139626895877No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG62580.1263261163803066No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61890.12493325891302615No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG55500.1120341875856027No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG52340.10565530411226028No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC49920.10077020980672588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61300.084.170161
GTATCAA161950.072.5747761
ATCAACG208650.055.9244543
CAACGCA208750.055.669065
TCAACGC211100.055.2753944
AACGCAG218050.053.342856
ACGCAGA238400.048.839387
CGCAGAG240200.048.4486168
TATCAAC253250.046.4280052
GCAGAGT258550.045.056119
GCAGCGC6350.042.1623048
GTCGTGC3600.036.3680231
TGGTCCT21700.035.64695
CGCCGAG851.7120274E-434.9974678
TGCAGCG7950.032.9284367
CTAGCCT41400.031.9076754
TGGTATC20400.031.5018562
CAGAGTA272650.030.66979410-11
GCCTTTC37150.029.9480327
AGAGTAC278400.029.8119610-11
Produced by FastQC (version 0.11.5)