FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4946664
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389430.7872578367966775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192470.38909050624825137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147850.298888301287494No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA80300.16233162389844955No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC73570.14872649527034784No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC66970.1353841700184205No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG66590.1346159755342186No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT63410.12818740064010817No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG62180.1257008763886126No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG59530.12034373064352057No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG54150.10946771399876765No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51690.10449466549577655No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC50880.10285719830576728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56250.082.125651
GTATCAA158200.074.904771
ATCAACG209350.056.357793
CAACGCA210000.056.149345
TCAACGC211650.055.8015754
GCAGCGC5950.053.991878
AACGCAG219000.053.8679126
ACGCAGA240200.049.1383137
CGCAGAG241200.048.909928
TATCAAC258950.045.9075162
GCAGAGT261350.045.207279
TGCAGCG7300.044.007077
CCGTGCG905.164282E-639.660699
ACGGGAC3400.036.748923
TGGTCCT24900.036.5554735
GTCGTGC4200.035.480551
TGGTATC21350.034.8350452
GTGGTAT22950.034.8034021
CGGTATC1451.0993426E-732.8266262
GCTCTAG9250.031.5757711
Produced by FastQC (version 0.11.5)