FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4913549
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231800.471756768885382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128040.26058557673893146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114670.2333751021919187No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56240.11445901933612547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.060.2181631
GTATCAA122000.054.5217321
ATCAACG157900.050.6382033
TCAACGC158400.050.553484
CAACGCA159650.050.1204035
AACGCAG164900.048.5241976
TATCAAC170150.047.342142
ACGCAGA193600.041.3000567
AGCGCGA1309.1858965E-1041.1895331
CGCAGAG195450.040.9391638
GCAGAGT225000.035.721139
ATACGGG5950.032.9977761
AGAGTAC219000.027.3414810-11
GAGTACT196100.025.22533612-13
CAGAGTA237800.025.09235610-11
AAGTACG4800.024.7899931
TCTGTCG12150.023.9923138
CTGTCGC12550.023.7016539
AGTACGG46300.023.38528812-13
GAGTACG44550.022.70144812-13
Produced by FastQC (version 0.11.5)