FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4913549
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA41500.039.1225135
TCAACGC44500.036.8859754
AACGCAG43800.036.6406176
ATCAACG45300.035.9719963
GTCTCGC11750.035.4254041
CGCAGAG45950.034.6671338
ACGCAGA46350.034.624087
GTATCAA53700.033.552351
CGCTCTG11750.029.8625135
CTCGCTC12800.029.7359753
TCGCTCT14100.028.6815074
CTGTCGC15150.028.6412649
TCTGTCG15000.026.9455058
TCTCGCT15500.026.4746112
TATCAAC66050.026.2919162
GGTATCA35150.025.0375021
AGTACGG49550.023.15202312-13
GAGTACG49400.022.92151612-13
GCAGAGT70450.022.6962039
CGCCGTT1354.5587512E-822.03339118-119
Produced by FastQC (version 0.11.5)