FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4883546
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225980.4627375271984742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128220.2625551187600158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116110.23775756386854963No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55940.11454791252094278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.060.110151
GTATCAA123450.052.43821
ATCAACG160000.048.3057863
TCAACGC161550.047.8049964
CAACGCA161900.047.51795
AACGCAG167600.045.936876
TATCAAC171900.044.857922
ACGCAGA194350.039.5223547
CGCAGAG195000.039.4207238
GCAGAGT227450.033.8227779
TCTGTCG11450.032.736468
CTGTCGC12300.031.4416229
ATACGGG6400.030.6791951
AGCGCGA800.005364309529.7495231
AGAGTAC219200.027.02074810-11
AAGTACG4600.025.8691521
CAGAGTA236500.024.70455410-11
GAGTACT197650.024.39792412-13
GAGTACG43750.023.32291212-13
CTCGCTC9900.022.8380183
Produced by FastQC (version 0.11.5)