FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857988
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218690.4501657887998076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120930.2489302155542583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111740.23001291892857703No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64500.1327710154903635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57300.058.3715021
GTATCAA122150.052.0351641
ATCAACG157200.049.8865743
TCAACGC158650.049.4681324
CAACGCA160900.048.73945
AACGCAG166550.047.1183056
TATCAAC171400.045.7536132
CGCAGAG190750.041.1405148
ACGCAGA191200.041.043697
GCAGAGT221900.035.472529
ATACGGG6550.035.4358181
CTGTCGC14200.034.3569839
TCTGTCG13850.033.506918
CGCTCTG9700.028.2167435
AGAGTAC217350.027.27766210-11
CTCGCTC9900.026.4446773
CAGAGTA233550.025.75495510-11
GAGTACT194250.025.63621512-13
TCGCTCT12800.024.1720894
AAGTACG5250.023.80561
Produced by FastQC (version 0.11.5)