FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857988
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA37600.037.6389275
TCAACGC40150.035.8408164
AACGCAG40250.035.5956046
ATCAACG41850.034.6690943
ACGCAGA42600.033.4924437
CGCAGAG42200.033.244018
GTATCAA49200.031.6630671
TCTGTCG12950.030.7552268
CTGTCGC13800.029.2922469
CGCTCTG9850.028.3732915
GTCTCGC10850.027.400411
CTCGCTC10950.025.5230083
TATCAAC59950.024.6978022
GGTATCA32000.023.7835561
GAGTACG45450.022.6323171
AGTACGG46750.022.44268212-13
TCTCGCT13950.022.1654852
TCGCTCT12100.022.1144054
GTACGGG56450.021.9086781
GCAGAGT68150.020.5858579
Produced by FastQC (version 0.11.5)