FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942355
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276230.558903599599786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149720.302932508895051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124050.2509937064415648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60700.059.904651
GTATCAA137450.059.5016141
ATCAACG170550.050.9328583
TCAACGC170600.050.9179274
CAACGCA171750.050.4338345
AACGCAG176400.049.1028756
TATCAAC181500.047.925612
ACGCAGA202800.042.710797
CGCAGAG203900.042.4220168
GCAGAGT234450.036.894229
TCTGTCG12650.029.6274438
AGAGTAC227050.029.14893310-11
ATCGTCG850.00721854227.9953068
CTCGCTC10650.026.815663
TCGCGTA450.00953125826.44509580-81
CTGTCGC13900.026.15981
CAGAGTA245550.025.75357210-11
GTCTCGC10050.024.9116541
GAGTACT211000.024.78281412-13
AGTACGG46850.024.63407712-13
Produced by FastQC (version 0.11.5)