FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942355
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA39650.040.4127045
AACGCAG41300.038.510376
TCAACGC42200.038.251974
ATCAACG43700.036.937853
ACGCAGA44750.035.674037
CGCAGAG45050.035.305098
TCTGTCG14250.033.7339748
GTATCAA54150.032.32951
CTGTCGC14900.031.86479
GTCTCGC11400.029.67191
CTCGCTC11100.027.8012623
TATCAAC63700.026.4583662
CGCTCTG12700.026.1686695
GGTATCA31600.024.9769781
TCGCTCT14500.024.9665874
TCTCGCT13800.023.2219582
GCAGAGT69950.023.1622669
GAGTACG48650.022.9324441
AGTACGG49750.022.54764612-13
TACGGGT8400.021.9017964
Produced by FastQC (version 0.11.5)