FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225518
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315510.6037870312569968No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160620.30737622566796247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144630.2767763884843569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141600.061.4270821
GGTATCA63500.057.995291
TCAACGC175650.051.007734
ATCAACG176650.050.7863353
CAACGCA179700.049.924355
AACGCAG184050.048.809046
TATCAAC191050.047.0829772
CGCAGAG206800.043.4387478
ACGCAGA207050.043.3296627
GCAGAGT242150.037.195669
AGAGTAC236200.030.02252610-11
GTCTCGC5850.025.424941
CAGAGTA255400.024.93541710-11
AGTACTT241800.024.57876612-13
GAGTACT220700.024.30044712-13
AGTACGG47350.024.06022512-13
GCGTATC2454.3557338E-721.855071
GAGTACG45800.021.62716712-13
TACGGGG26700.019.8306942
GTACGGG55850.019.7071741
Produced by FastQC (version 0.11.5)