FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5177710
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310130.598971359925527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157870.30490313285216825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142890.2759714236602668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137400.062.8526761
GGTATCA60100.057.140551
TCAACGC172250.050.7137834
ATCAACG172550.050.6256143
CAACGCA173650.050.1326145
AACGCAG177950.048.8811576
TATCAAC189550.046.210772
CGCAGAG197800.043.9456638
ACGCAGA198750.043.735617
GCAGAGT233100.037.494859
AGAGTAC226900.030.70538110-11
AGTACTT234300.025.2668612-13
CAGAGTA245750.025.00914610-11
AGTACGG45950.024.9937232
GAGTACG45800.024.8205281
GAGTACT208750.024.7539112-13
GTCTCGC5550.021.447671
GTACGGG54300.020.6023713
TACGGGA23450.020.3004824
ACGACCG1251.2637598E-519.03909368-69
Produced by FastQC (version 0.11.5)