FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5177710
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA36450.042.585735
TCAACGC37800.041.0648164
AACGCAG37950.041.0441366
CGCAGAG39700.039.6837588
ACGCAGA39800.039.585587
ATCAACG39700.039.2496833
GTATCAA54350.032.0584871
GTCTCGC5650.027.3652781
CGATACC8750.024.495134118-119
AGTACGG45000.024.37434212-13
GCAGAGT66550.023.9411039
TATCAAC67900.023.824542
GAGTACG45300.022.90056812-13
GGTATCA33750.020.9675731
CTGTCGC8250.020.8978449
AGAGTAC58450.020.29138210-11
GTACGGG54100.019.6757261
GTATACG5300.019.0742741
TACGGGT8750.019.0314184
ACGGGAC4458.0035534E-1118.7108443
Produced by FastQC (version 0.11.5)