FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191855
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319110.6146358093590826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150000.2889140779162746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147690.28446480111636396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60900.062.256831
GTATCAA142200.061.8776661
TCAACGC172000.050.8894124
ATCAACG173000.050.6296463
CAACGCA174200.050.238495
AACGCAG179150.048.913976
TATCAAC191850.045.9342352
ACGCAGA202950.043.1485067
CGCAGAG203000.043.137888
GCAGAGT239000.036.789479
AGAGTAC231650.030.21388610-11
CGACGCG400.005368391529.74520514-15
CGCGCAA450.00952022326.45139168-69
CAGAGTA250050.025.20699510-11
AGTACTT237800.025.07953812-13
GAGTACT216150.024.60533112-13
GAGTACG43100.024.2051581
AGTACGG44250.024.19948812-13
GTACGGG54050.022.7205411
TACGGGG25750.022.4149462
Produced by FastQC (version 0.11.5)