FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191855
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA34350.044.231375
TCAACGC35100.043.2862554
AACGCAG36850.041.23026
CGCAGAG37900.040.0879448
ATCAACG38050.040.0847243
ACGCAGA38850.039.2604337
GTATCAA53600.032.441051
CTGTCGC8700.027.2879249
TCTGTCG9450.027.0053398
GTCTCGC7600.025.7686961
TATCAAC64100.025.0906372
AGTACGG43000.024.15573512-13
GCAGAGT65200.023.7586989
GAGTACG42600.023.61624112-13
CTCGCTC7950.023.1416873
TCGCTCT9900.021.5816424
CGATACC8600.020.829338118-119
CGCTCTG9100.020.2179665
GTACGGG51200.020.0524861
AGAGTAC57850.019.54510910-11
Produced by FastQC (version 0.11.5)