FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5192794
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA33800.038.8893785
CGCAGAG34950.036.9087378
TCAACGC36600.036.402114
ACGCAGA35650.036.0155377
AACGCAG36600.035.4072386
ATCAACG38450.034.6506463
GTCTCGC9100.028.7563131
TGCGGAC1704.4648004E-727.9747419
GTATCAA49850.027.9172481
TCTGTCG13800.026.7072548
CTCGCTC9650.026.5033513
CTGTCGC13350.025.3815759
TATCAAC56550.024.7167112
CGCTCTG10150.024.0255455
ACGGGAC4000.023.791383
GTACGGG47100.023.7388081
TCGCTCT12300.022.727544
AGTACGG37900.022.42972412-13
GAGTACG37750.022.3714081
TACGGGA22100.022.0687142
Produced by FastQC (version 0.11.5)