FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5172871
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392590.7589402480750052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180710.34934178718162506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155540.30068408819783055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA155200.065.262111
GGTATCA66600.062.7272381
ATCAACG189850.054.535783
TCAACGC189700.054.516174
CAACGCA189450.054.461955
AACGCAG195000.053.2108466
TATCAAC200000.051.94662
ACGCAGA210650.049.2858547
CGCAGAG211100.049.1807868
GCAGAGT243500.042.637239
CGCGTGA758.184157E-539.6736341
CTCGCTC9150.036.4174773
GTCTCGC10400.034.905171
AGAGTAC234900.032.49646810-11
TCTGTCG10750.029.88648
TCTCGCT11700.029.4974882
TCGCTCT11700.028.988914
GAGTACT223550.028.86367612-13
CAGAGTA253500.028.79778710-11
AGTACTT244950.027.50790212-13
Produced by FastQC (version 0.11.5)