FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5172871
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA31500.040.4104235
AACGCAG34200.038.419016
ACGCAGA34600.037.4600877
CGCAGAG34450.037.447358
TCAACGC35700.035.989494
GTCTCGC9500.033.807251
ATCAACG38350.033.5022773
TCTGTCG11350.031.4272218
CTCGCTC9600.030.360633
GTATCAA48500.029.6765461
TCGCTCT11750.028.8554044
CGCTCTG10750.028.7729935
CTGTCGC12800.027.401869
TCTCGCT12250.025.7350922
AGTACGG36900.024.80923812-13
GTACGGG45400.024.1046961
TACGGGA20400.023.9094522
GTTCGAC1751.7578346E-523.7902871
TATCAAC57600.023.5449772
GAGTACG36950.021.8909281
Produced by FastQC (version 0.11.5)