FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5168746
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383840.7426172615175906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184660.35726267067486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150920.2919857156842298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA158400.065.544041
GGTATCA68800.062.1470221
TCAACGC194250.054.007664
ATCAACG194700.053.8522723
CAACGCA195100.053.789855
AACGCAG199300.052.654266
TATCAAC205750.051.1336172
ACGCAGA216400.048.466017
CGCAGAG216950.048.3431438
GCAGAGT250950.041.9355969
GTCTCGC8650.038.6065941
TCTGTCG9750.036.6090858
CTGTCGC10200.036.1604429
CTCGCTC9800.032.182063
AGAGTAC242150.032.17090210-11
CGCTCTG10700.030.0240335
TCTCGCT11900.029.50322
GAGTACT228100.029.04070712-13
CAGAGTA259200.028.47108510-11
AGTACTT248850.027.9698712-13
Produced by FastQC (version 0.11.5)