FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7652640
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488370.638171924982751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311300.40678772292960336No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184210.24071431558259632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171540.2241579376528884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA401.9032086E-889.2408452
GGTATCA118150.075.231161
GTATCAA270800.058.5942841
GTCTCGC51800.056.2791141
CTCGCTC49850.055.495093
TCTGTCG53250.055.1895758
TCTCGCT54200.053.5664632
ACGGGAC16050.052.635663
TATAACG2900.051.287842
CGCTCTG51200.051.12695
ATCAACG334050.047.320233
GCGTACT4300.047.0426061
CAACGCA336850.046.909225
TCAACGC342100.046.3632434
GCGTAAC1800.046.2739371
AACGCAG349000.045.479816
TGCGGAC5500.045.429339
GCGCAGA7100.045.2497671
CGTACTT4350.045.13332
GCGTAAG6900.043.974611
Produced by FastQC (version 0.11.5)