FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7652640
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA243390.31804710531267644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC48600.056.0454061
CTCGCTC45750.054.9909673
TCTCGCT51100.052.3761672
GGTATCA64450.052.1358341
TCTGTCG51400.050.3146868
CGCTCTG48450.049.2254685
ACGGGAC18350.046.6732983
GTATCAA139050.046.5765881
GTACGGG339800.043.264921
CAACGCA149000.042.1119585
TGCGGAC5650.042.0895739
ATCAACG156200.040.4756973
AACGCAG155150.040.2716756
TCGCTCT68700.040.256454
ACGCAGA155400.040.129857
TCAACGC158650.039.8503764
CGCAGAG157300.039.7986458
GGCTAGA40250.039.7463461
GTCTCAC75200.039.621481
ACGGGTC8800.037.8483923
Produced by FastQC (version 0.11.5)