FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7646852
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488720.6391126701549866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311160.40691254388080217No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184320.24104036536865106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173830.2273223020401075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123650.072.327321
GCGTAAC2000.059.502811
GTATCAA281950.059.154591
GCGTACT4400.058.1504751
TCTGTCG50850.057.800788
GTCTCGC48100.057.152381
CTCGCTC46600.055.1614043
TCTCGCT49000.053.9168322
GCGCAGA5450.052.4061471
CGCTCTG47400.050.7134865
TGCGGAC5250.049.8644529
CGTACTT4550.049.6946532
ACGGGAC16100.049.154493
ATCAACG340050.048.977613
CAACGCA341750.048.940995
TAACGCA3900.048.8212174
TCAACGC347500.048.2171064
AACGCAG354900.047.37666
CGTAACG1056.3901098E-945.3354762
TATCAAC370050.044.7497142
Produced by FastQC (version 0.11.5)