FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7635577
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487570.6385503020924287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313640.41076136092923954No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184040.24102959082201647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171030.22399093087529598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125100.072.973151
GCGTAAC2350.060.776891
GCGTACT4050.060.2454071
TCTGTCG51300.059.6118748
CGTACGA1100.059.5036932
GTATCAA279600.058.148521
GTCTCGC48200.055.4363171
CTCGCTC46450.052.9064033
TCTCGCT50550.052.2643662
TGCGGAC4650.051.1792689
CGCTCTG46950.050.059715
CGTACTT4600.049.1552242
ATCAACG342450.047.4882743
CAACGCA346400.046.8418545
GAGTCGG12750.046.6750641
TCAACGC351700.046.3408054
GGCTAGA43250.045.544611
AACGCAG359250.045.3278436
ACGGGAC15250.044.8716353
GCGTAAG4950.044.4827541
Produced by FastQC (version 0.11.5)