FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7635577
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA253890.33250925241144186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG52750.055.9013028
GTCTCGC46550.053.7850721
CTCGCTC45450.052.0837973
GGTATCA64200.051.133251
TCTCGCT49100.050.3917662
CGCTCTG47350.047.481595
GTATCAA134500.047.3550761
ACGGGAC18750.044.727163
TGCGGAC4700.044.270359
GTACGGG335250.043.531651
CAACGCA146700.042.2059865
GTCTCAC73900.041.041681
ACGGGTC7950.040.399883
AACGCAG154350.040.176466
ACGCAGA154250.040.125427
ATCAACG154750.040.087333
CGCAGAG156150.039.9769868
TCAACGC157000.039.5885964
GGCTAGA40550.038.131321
TCGCTCT67100.037.9380234
Produced by FastQC (version 0.11.5)