FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7748672
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA291720.3764774144524378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG55200.054.071878
GTCTCGC48900.052.9064871
GGTATCA66700.052.5766561
CTCGCTC48150.049.4179763
TCTCGCT51950.048.5445252
CGCTCTG49500.044.7148485
GTATCAA137800.043.3191641
GTACGGG332250.041.80881
GTCTCAC78100.041.02741
ACGGGAC18150.040.8667683
CAACGCA146150.039.059295
GGCTAGA41850.038.4242251
GAGTACG218050.037.5538331
AACGCAG153400.037.406446
ACGCAGA154150.037.185477
TCTGTCA79800.037.180028
ATCAACG155300.037.139133
CGCAGAG155000.037.020078
TCAACGC157550.036.4964524
TCGCTCT72700.036.3220944
Produced by FastQC (version 0.11.5)