FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8815957
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680350.7717256334167691No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325860.36962521482352967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283500.3215759786487162No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101760.11542706027263971No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT99770.11316978973468224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88760.10068107183372152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116650.076.610991
GTATCAA341100.063.7546041
ATCAACG398350.054.4705853
TCAACGC400300.054.309284
CAACGCA404550.053.7093245
AACGCAG420100.051.7619976
TATCAAC443400.049.204672
ACGCAGA509450.042.917277
CGCAGAG516500.042.3405888
TCTGTCG21800.040.934218
GCAGAGT553450.039.5890439
GTCTCGC22800.035.4903221
GAATACG40850.033.0586789
AGAGTAC506300.031.73718310-11
CAGAGTA521300.029.52870210-11
GTGCTAG52250.028.8097921
GAGTACT407650.026.1518412-13
TCTCGCT32200.025.8680272
AGTACGG195550.025.85906212-13
AGTACTT429100.025.79424712-13
Produced by FastQC (version 0.11.5)